bcl 2 mab Search Results


bcl 2  (Sino Biological)
93
Sino Biological bcl 2
Bcl 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/Bcl-2+Antibody%2C+Rabbit+MAb/pmc09214597-48-59-56
Average 93 stars, based on 1 article reviews
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bcl 2  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc bcl 2
Bcl 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/Bcl-2+Mouse+mAb/pmc10573393-262-5-15
Average 96 stars, based on 1 article reviews
bcl 2 - by Bioz Stars, 2026-11
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anti phospho bcl 2  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc anti phospho bcl 2
Anti Phospho Bcl 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/Phospho-Bcl-2+(Ser70)+Rabbit+mAb/10__2147_slash_ott__s267734-99-186-192
Average 94 stars, based on 1 article reviews
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bcl 2  (ABclonal Biotechnology)
98
ABclonal Biotechnology bcl 2
1,2-DCE exposure induces cognitive impairment and hippocampal neuronal apoptosis in mice. ( A ) Escape latency during MWM training. n = 5. ( B ) Proportion of time spent in the target quadrant during the probe trial. n = 5. ( C ) Representative trajectory (red lines) images from the probe test. ( D ) RI in the training phase. n = 5. ( E ) RI in the test phase. n = 5. ( F ) DI in the test phase. n = 5. ( G ) Body weight and brain weight of mice. n = 5. ( H ) Ultrastructure of hippocampal CA1 neurons. n = 3. Scale bar: 2 µm; mitochondria (red arrows). ( I ) ATP levels in hippocampal tissues. n = 5. ( J ) Representative western blot bands of BAX and <t>Bcl-2.</t> ( K ) Quantitative analysis of the Bcl-2/Bax ratio. n = 5. *, vs. control; &, vs. 0.225 g/m 3 ; #, vs. 0.45 g/m 3 , p < 0.05.
Bcl 2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/Bcl-2+Rabbit+mAb/pmc13604869-86-16-19
Average 98 stars, based on 1 article reviews
bcl 2 - by Bioz Stars, 2026-11
98/100 stars
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bcl xl rabbit mab a23766 abclonal wb  (ABclonal Biotechnology)
93
ABclonal Biotechnology bcl xl rabbit mab a23766 abclonal wb
1,2-DCE exposure induces cognitive impairment and hippocampal neuronal apoptosis in mice. ( A ) Escape latency during MWM training. n = 5. ( B ) Proportion of time spent in the target quadrant during the probe trial. n = 5. ( C ) Representative trajectory (red lines) images from the probe test. ( D ) RI in the training phase. n = 5. ( E ) RI in the test phase. n = 5. ( F ) DI in the test phase. n = 5. ( G ) Body weight and brain weight of mice. n = 5. ( H ) Ultrastructure of hippocampal CA1 neurons. n = 3. Scale bar: 2 µm; mitochondria (red arrows). ( I ) ATP levels in hippocampal tissues. n = 5. ( J ) Representative western blot bands of BAX and <t>Bcl-2.</t> ( K ) Quantitative analysis of the Bcl-2/Bax ratio. n = 5. *, vs. control; &, vs. 0.225 g/m 3 ; #, vs. 0.45 g/m 3 , p < 0.05.
Bcl Xl Rabbit Mab A23766 Abclonal Wb, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/Bcl-XL+Rabbit+mAb/pmc11650350__tau___13___11___2553___supplementary-3-89-93
Average 93 stars, based on 1 article reviews
bcl xl rabbit mab a23766 abclonal wb - by Bioz Stars, 2026-11
93/100 stars
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anti human bcl2 af647  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc anti human bcl2 af647
1,2-DCE exposure induces cognitive impairment and hippocampal neuronal apoptosis in mice. ( A ) Escape latency during MWM training. n = 5. ( B ) Proportion of time spent in the target quadrant during the probe trial. n = 5. ( C ) Representative trajectory (red lines) images from the probe test. ( D ) RI in the training phase. n = 5. ( E ) RI in the test phase. n = 5. ( F ) DI in the test phase. n = 5. ( G ) Body weight and brain weight of mice. n = 5. ( H ) Ultrastructure of hippocampal CA1 neurons. n = 3. Scale bar: 2 µm; mitochondria (red arrows). ( I ) ATP levels in hippocampal tissues. n = 5. ( J ) Representative western blot bands of BAX and <t>Bcl-2.</t> ( K ) Quantitative analysis of the Bcl-2/Bax ratio. n = 5. *, vs. control; &, vs. 0.225 g/m 3 ; #, vs. 0.45 g/m 3 , p < 0.05.
Anti Human Bcl2 Af647, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/Bcl-2+Mouse+mAb/pmc10236156-275-36-39
Average 94 stars, based on 1 article reviews
anti human bcl2 af647 - by Bioz Stars, 2026-11
94/100 stars
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mcl 1  (ABclonal Biotechnology)
91
ABclonal Biotechnology mcl 1
1,2-DCE exposure induces cognitive impairment and hippocampal neuronal apoptosis in mice. ( A ) Escape latency during MWM training. n = 5. ( B ) Proportion of time spent in the target quadrant during the probe trial. n = 5. ( C ) Representative trajectory (red lines) images from the probe test. ( D ) RI in the training phase. n = 5. ( E ) RI in the test phase. n = 5. ( F ) DI in the test phase. n = 5. ( G ) Body weight and brain weight of mice. n = 5. ( H ) Ultrastructure of hippocampal CA1 neurons. n = 3. Scale bar: 2 µm; mitochondria (red arrows). ( I ) ATP levels in hippocampal tissues. n = 5. ( J ) Representative western blot bands of BAX and <t>Bcl-2.</t> ( K ) Quantitative analysis of the Bcl-2/Bax ratio. n = 5. *, vs. control; &, vs. 0.225 g/m 3 ; #, vs. 0.45 g/m 3 , p < 0.05.
Mcl 1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/MCL1+Rabbit+mAb/pm34417569__41422_2021_533_MOESM1_ESM-18-0-15
Average 91 stars, based on 1 article reviews
mcl 1 - by Bioz Stars, 2026-11
91/100 stars
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mouse anti bcl 2  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc mouse anti bcl 2
1,2-DCE exposure induces cognitive impairment and hippocampal neuronal apoptosis in mice. ( A ) Escape latency during MWM training. n = 5. ( B ) Proportion of time spent in the target quadrant during the probe trial. n = 5. ( C ) Representative trajectory (red lines) images from the probe test. ( D ) RI in the training phase. n = 5. ( E ) RI in the test phase. n = 5. ( F ) DI in the test phase. n = 5. ( G ) Body weight and brain weight of mice. n = 5. ( H ) Ultrastructure of hippocampal CA1 neurons. n = 3. Scale bar: 2 µm; mitochondria (red arrows). ( I ) ATP levels in hippocampal tissues. n = 5. ( J ) Representative western blot bands of BAX and <t>Bcl-2.</t> ( K ) Quantitative analysis of the Bcl-2/Bax ratio. n = 5. *, vs. control; &, vs. 0.225 g/m 3 ; #, vs. 0.45 g/m 3 , p < 0.05.
Mouse Anti Bcl 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/Bcl-2+Mouse+mAb/pmc12133564-78-49-55
Average 93 stars, based on 1 article reviews
mouse anti bcl 2 - by Bioz Stars, 2026-11
93/100 stars
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b cell lymphoma 2  (Sino Biological)
93
Sino Biological b cell lymphoma 2
1,2-DCE exposure induces cognitive impairment and hippocampal neuronal apoptosis in mice. ( A ) Escape latency during MWM training. n = 5. ( B ) Proportion of time spent in the target quadrant during the probe trial. n = 5. ( C ) Representative trajectory (red lines) images from the probe test. ( D ) RI in the training phase. n = 5. ( E ) RI in the test phase. n = 5. ( F ) DI in the test phase. n = 5. ( G ) Body weight and brain weight of mice. n = 5. ( H ) Ultrastructure of hippocampal CA1 neurons. n = 3. Scale bar: 2 µm; mitochondria (red arrows). ( I ) ATP levels in hippocampal tissues. n = 5. ( J ) Representative western blot bands of BAX and <t>Bcl-2.</t> ( K ) Quantitative analysis of the Bcl-2/Bax ratio. n = 5. *, vs. control; &, vs. 0.225 g/m 3 ; #, vs. 0.45 g/m 3 , p < 0.05.
B Cell Lymphoma 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl+2+mab/BCL2+%2F+Bcl-2+Antibody%2C+Mouse+MAb/pmc08752210-58-37-43
Average 93 stars, based on 1 article reviews
b cell lymphoma 2 - by Bioz Stars, 2026-11
93/100 stars
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Image Search Results


1,2-DCE exposure induces cognitive impairment and hippocampal neuronal apoptosis in mice. ( A ) Escape latency during MWM training. n = 5. ( B ) Proportion of time spent in the target quadrant during the probe trial. n = 5. ( C ) Representative trajectory (red lines) images from the probe test. ( D ) RI in the training phase. n = 5. ( E ) RI in the test phase. n = 5. ( F ) DI in the test phase. n = 5. ( G ) Body weight and brain weight of mice. n = 5. ( H ) Ultrastructure of hippocampal CA1 neurons. n = 3. Scale bar: 2 µm; mitochondria (red arrows). ( I ) ATP levels in hippocampal tissues. n = 5. ( J ) Representative western blot bands of BAX and Bcl-2. ( K ) Quantitative analysis of the Bcl-2/Bax ratio. n = 5. *, vs. control; &, vs. 0.225 g/m 3 ; #, vs. 0.45 g/m 3 , p < 0.05.

Journal: Biology

Article Title: 1,2-Dichloroethane Induces Neuronal Apoptosis Through AMPK-mTOR-ULK1 Pathway Activation and Autophagic Flux Impairment

doi: 10.3390/biology15181580

Figure Lengend Snippet: 1,2-DCE exposure induces cognitive impairment and hippocampal neuronal apoptosis in mice. ( A ) Escape latency during MWM training. n = 5. ( B ) Proportion of time spent in the target quadrant during the probe trial. n = 5. ( C ) Representative trajectory (red lines) images from the probe test. ( D ) RI in the training phase. n = 5. ( E ) RI in the test phase. n = 5. ( F ) DI in the test phase. n = 5. ( G ) Body weight and brain weight of mice. n = 5. ( H ) Ultrastructure of hippocampal CA1 neurons. n = 3. Scale bar: 2 µm; mitochondria (red arrows). ( I ) ATP levels in hippocampal tissues. n = 5. ( J ) Representative western blot bands of BAX and Bcl-2. ( K ) Quantitative analysis of the Bcl-2/Bax ratio. n = 5. *, vs. control; &, vs. 0.225 g/m 3 ; #, vs. 0.45 g/m 3 , p < 0.05.

Article Snippet: The primary antibodies used in this study were as follows: Bax (1:1000, A20227, ABclonal, Wuhan, China), Bcl-2 (1:1000, A19693, ABclonal), cleaved caspase 3 (1:1000, YM343, Immunoway, San Jose, CA, USA), LC3B (1:1000, A19665, ABclonal), Beclin 1 (1:1000, A21191, ABclonal), p62 (1:1000, WL02385, Wanleibio, Shenyang, China), lysosome-associated membrane protein 1 (LAMP1, 1:1000, A23947, ABclonal), cathepsin D (CTSD, 1:1000, 74089, Cell Signaling Technology, Danvers, MA, USA), p-AMPK (Thr172, 1:1000, WL05103, Wanleibio), AMPK (1:1000, WL02254, Wanleibio), p-mTOR (Ser2448, 1:1000, AP0115, ABclonal), mTOR (1:1000, WL02477, Wanleibio), p-ULK1 (Ser555, 1:1000, YP1542, Immunoway), ULK1 (1:1000, WL03067, Wanleibio), GAPDH (1:2000, sc-32233, Santa Cruz, Dallas, TX, USA), and β-actin (1:2000, sc-47778, Santa Cruz).

Techniques: Western Blot, Analysis, Control

2-CE exposure induces mitochondrial damage and apoptosis in PC12 cells. ( A ) Cell morphology of PC12 cells. ( B ) Cell viability of PC12 cells. n = 6. ( C ) ATP levels in PC12 cells. n = 3. ( D ) ROS levels in PC12 cells. n = 3. ( E ) Changes in mitochondrial membrane potential in PC12 cells. CCCP was used as the positive control. ( F ) Representative western blot bands of BAX and Bcl-2. ( G ) Quantitative analysis of Bcl-2/BAX ratio. n = 3. *, vs. control; &, vs. 22.5 mM, p < 0.05.

Journal: Biology

Article Title: 1,2-Dichloroethane Induces Neuronal Apoptosis Through AMPK-mTOR-ULK1 Pathway Activation and Autophagic Flux Impairment

doi: 10.3390/biology15181580

Figure Lengend Snippet: 2-CE exposure induces mitochondrial damage and apoptosis in PC12 cells. ( A ) Cell morphology of PC12 cells. ( B ) Cell viability of PC12 cells. n = 6. ( C ) ATP levels in PC12 cells. n = 3. ( D ) ROS levels in PC12 cells. n = 3. ( E ) Changes in mitochondrial membrane potential in PC12 cells. CCCP was used as the positive control. ( F ) Representative western blot bands of BAX and Bcl-2. ( G ) Quantitative analysis of Bcl-2/BAX ratio. n = 3. *, vs. control; &, vs. 22.5 mM, p < 0.05.

Article Snippet: The primary antibodies used in this study were as follows: Bax (1:1000, A20227, ABclonal, Wuhan, China), Bcl-2 (1:1000, A19693, ABclonal), cleaved caspase 3 (1:1000, YM343, Immunoway, San Jose, CA, USA), LC3B (1:1000, A19665, ABclonal), Beclin 1 (1:1000, A21191, ABclonal), p62 (1:1000, WL02385, Wanleibio, Shenyang, China), lysosome-associated membrane protein 1 (LAMP1, 1:1000, A23947, ABclonal), cathepsin D (CTSD, 1:1000, 74089, Cell Signaling Technology, Danvers, MA, USA), p-AMPK (Thr172, 1:1000, WL05103, Wanleibio), AMPK (1:1000, WL02254, Wanleibio), p-mTOR (Ser2448, 1:1000, AP0115, ABclonal), mTOR (1:1000, WL02477, Wanleibio), p-ULK1 (Ser555, 1:1000, YP1542, Immunoway), ULK1 (1:1000, WL03067, Wanleibio), GAPDH (1:2000, sc-32233, Santa Cruz, Dallas, TX, USA), and β-actin (1:2000, sc-47778, Santa Cruz).

Techniques: Morphology, Membrane, Positive Control, Western Blot, Analysis, Control

3-MA intervention alleviates autophagosome accumulation and reduces apoptosis in 2-CE-treated PC12 cells. ( A ) Representative western blot bands of LC3-II/I, Beclin 1, p62, LAMP1 and CTSD. ( B ) Quantitative analysis of the LC3-II/I ratio. ( C ) Quantitative analysis of Beclin 1 expression. ( D ) Quantitative analysis of p62 expression. ( E ) Quantitative analysis of LAMP1 expression. ( F ) Quantitative analysis of CTSD expression. ( G ) Representative western blot bands of BAX and Bcl-2. ( H ) Quantitative analysis of the Bcl-2/BAX ratio. ( I ) Cell viability after 3-MA treatment. n = 3. *, vs. control; Δ, vs. 90 mM, p < 0.05.

Journal: Biology

Article Title: 1,2-Dichloroethane Induces Neuronal Apoptosis Through AMPK-mTOR-ULK1 Pathway Activation and Autophagic Flux Impairment

doi: 10.3390/biology15181580

Figure Lengend Snippet: 3-MA intervention alleviates autophagosome accumulation and reduces apoptosis in 2-CE-treated PC12 cells. ( A ) Representative western blot bands of LC3-II/I, Beclin 1, p62, LAMP1 and CTSD. ( B ) Quantitative analysis of the LC3-II/I ratio. ( C ) Quantitative analysis of Beclin 1 expression. ( D ) Quantitative analysis of p62 expression. ( E ) Quantitative analysis of LAMP1 expression. ( F ) Quantitative analysis of CTSD expression. ( G ) Representative western blot bands of BAX and Bcl-2. ( H ) Quantitative analysis of the Bcl-2/BAX ratio. ( I ) Cell viability after 3-MA treatment. n = 3. *, vs. control; Δ, vs. 90 mM, p < 0.05.

Article Snippet: The primary antibodies used in this study were as follows: Bax (1:1000, A20227, ABclonal, Wuhan, China), Bcl-2 (1:1000, A19693, ABclonal), cleaved caspase 3 (1:1000, YM343, Immunoway, San Jose, CA, USA), LC3B (1:1000, A19665, ABclonal), Beclin 1 (1:1000, A21191, ABclonal), p62 (1:1000, WL02385, Wanleibio, Shenyang, China), lysosome-associated membrane protein 1 (LAMP1, 1:1000, A23947, ABclonal), cathepsin D (CTSD, 1:1000, 74089, Cell Signaling Technology, Danvers, MA, USA), p-AMPK (Thr172, 1:1000, WL05103, Wanleibio), AMPK (1:1000, WL02254, Wanleibio), p-mTOR (Ser2448, 1:1000, AP0115, ABclonal), mTOR (1:1000, WL02477, Wanleibio), p-ULK1 (Ser555, 1:1000, YP1542, Immunoway), ULK1 (1:1000, WL03067, Wanleibio), GAPDH (1:2000, sc-32233, Santa Cruz, Dallas, TX, USA), and β-actin (1:2000, sc-47778, Santa Cruz).

Techniques: Western Blot, Analysis, Expressing, Control

Trehalose treatment restores autophagic flux and reduces apoptosis in 2-CE-treated PC12 cells. ( A ) Representative western blot bands of LC3-II/I, p62, LAMP1 and CTSD. ( B ) Quantitative analysis of the LC3-II/I ratio. ( C ) Quantitative analysis of p62 expression. ( D ) Quantitative analysis of LAMP1 expression. ( E ) Quantitative analysis of CTSD expression. ( F ) Representative western blot bands of BAX and Bcl-2. ( G ) Quantitative analysis of the Bcl-2/BAX ratio. n = 3. *, vs. control; Δ, vs. 90 mM, p < 0.05.

Journal: Biology

Article Title: 1,2-Dichloroethane Induces Neuronal Apoptosis Through AMPK-mTOR-ULK1 Pathway Activation and Autophagic Flux Impairment

doi: 10.3390/biology15181580

Figure Lengend Snippet: Trehalose treatment restores autophagic flux and reduces apoptosis in 2-CE-treated PC12 cells. ( A ) Representative western blot bands of LC3-II/I, p62, LAMP1 and CTSD. ( B ) Quantitative analysis of the LC3-II/I ratio. ( C ) Quantitative analysis of p62 expression. ( D ) Quantitative analysis of LAMP1 expression. ( E ) Quantitative analysis of CTSD expression. ( F ) Representative western blot bands of BAX and Bcl-2. ( G ) Quantitative analysis of the Bcl-2/BAX ratio. n = 3. *, vs. control; Δ, vs. 90 mM, p < 0.05.

Article Snippet: The primary antibodies used in this study were as follows: Bax (1:1000, A20227, ABclonal, Wuhan, China), Bcl-2 (1:1000, A19693, ABclonal), cleaved caspase 3 (1:1000, YM343, Immunoway, San Jose, CA, USA), LC3B (1:1000, A19665, ABclonal), Beclin 1 (1:1000, A21191, ABclonal), p62 (1:1000, WL02385, Wanleibio, Shenyang, China), lysosome-associated membrane protein 1 (LAMP1, 1:1000, A23947, ABclonal), cathepsin D (CTSD, 1:1000, 74089, Cell Signaling Technology, Danvers, MA, USA), p-AMPK (Thr172, 1:1000, WL05103, Wanleibio), AMPK (1:1000, WL02254, Wanleibio), p-mTOR (Ser2448, 1:1000, AP0115, ABclonal), mTOR (1:1000, WL02477, Wanleibio), p-ULK1 (Ser555, 1:1000, YP1542, Immunoway), ULK1 (1:1000, WL03067, Wanleibio), GAPDH (1:2000, sc-32233, Santa Cruz, Dallas, TX, USA), and β-actin (1:2000, sc-47778, Santa Cruz).

Techniques: Western Blot, Analysis, Expressing, Control

AMPK-mTOR-ULK1 signaling mediates 2-CE-induced autophagy and subsequent apoptosis in PC12 cells. ( A ) Representative western blot bands of p-AMPK, AMPK, p-mTOR, mTOR, p-ULK1 and ULK1. ( B ) Quantitative analysis of the p-AMPK/AMPK ratio. ( C ) Quantitative analysis of the p-mTOR/mTOR ratio. ( D ) Quantitative analysis of the p-ULK1/ULK1 ratio. ( E ) Representative western blot bands of LC3-II/I, Beclin 1, p62, LAMP1 and CTSD. ( F ) Quantitative analysis of the LC3-II/I ratio. ( G ) Quantitative analysis of Beclin 1 expression. ( H ) Quantitative analysis of p62 expression. ( I ) Quantitative analysis of LAMP1 expression. ( J ) Quantitative analysis of CTSD expression. ( K ) Representative fluorescence images of autophagic flux. ( L ) Representative western blot bands of BAX and Bcl-2. ( M ) Quantitative analysis of the Bcl-2/BAX ratio. n = 3. *, vs. control; Δ, vs. 90 mM, p < 0.05.

Journal: Biology

Article Title: 1,2-Dichloroethane Induces Neuronal Apoptosis Through AMPK-mTOR-ULK1 Pathway Activation and Autophagic Flux Impairment

doi: 10.3390/biology15181580

Figure Lengend Snippet: AMPK-mTOR-ULK1 signaling mediates 2-CE-induced autophagy and subsequent apoptosis in PC12 cells. ( A ) Representative western blot bands of p-AMPK, AMPK, p-mTOR, mTOR, p-ULK1 and ULK1. ( B ) Quantitative analysis of the p-AMPK/AMPK ratio. ( C ) Quantitative analysis of the p-mTOR/mTOR ratio. ( D ) Quantitative analysis of the p-ULK1/ULK1 ratio. ( E ) Representative western blot bands of LC3-II/I, Beclin 1, p62, LAMP1 and CTSD. ( F ) Quantitative analysis of the LC3-II/I ratio. ( G ) Quantitative analysis of Beclin 1 expression. ( H ) Quantitative analysis of p62 expression. ( I ) Quantitative analysis of LAMP1 expression. ( J ) Quantitative analysis of CTSD expression. ( K ) Representative fluorescence images of autophagic flux. ( L ) Representative western blot bands of BAX and Bcl-2. ( M ) Quantitative analysis of the Bcl-2/BAX ratio. n = 3. *, vs. control; Δ, vs. 90 mM, p < 0.05.

Article Snippet: The primary antibodies used in this study were as follows: Bax (1:1000, A20227, ABclonal, Wuhan, China), Bcl-2 (1:1000, A19693, ABclonal), cleaved caspase 3 (1:1000, YM343, Immunoway, San Jose, CA, USA), LC3B (1:1000, A19665, ABclonal), Beclin 1 (1:1000, A21191, ABclonal), p62 (1:1000, WL02385, Wanleibio, Shenyang, China), lysosome-associated membrane protein 1 (LAMP1, 1:1000, A23947, ABclonal), cathepsin D (CTSD, 1:1000, 74089, Cell Signaling Technology, Danvers, MA, USA), p-AMPK (Thr172, 1:1000, WL05103, Wanleibio), AMPK (1:1000, WL02254, Wanleibio), p-mTOR (Ser2448, 1:1000, AP0115, ABclonal), mTOR (1:1000, WL02477, Wanleibio), p-ULK1 (Ser555, 1:1000, YP1542, Immunoway), ULK1 (1:1000, WL03067, Wanleibio), GAPDH (1:2000, sc-32233, Santa Cruz, Dallas, TX, USA), and β-actin (1:2000, sc-47778, Santa Cruz).

Techniques: Western Blot, Analysis, Expressing, Fluorescence, Control